Estradiol enanthate injections – administration

Estradiol enanthate injections are a form of estrogen hormone therapy commonly used in gender-affirming care for transgender women or individuals assigned male at birth who are seeking feminization. Estradiol is the primary form of estrogen found in the human body and plays a key role in the development of secondary sexual characteristics typically associated with females. Here’s a guide on how to take estradiol enanthate injections:


The dosage of estradiol enanthate injections varies depending on individual needs, hormone levels, treatment goals, and healthcare provider recommendations.

Typical dosages range from 5 to 20 mg administered intramuscularly every one to two weeks.



Before administering the injection, gather all necessary supplies, including the vial of estradiol enanthate, a syringe, a needle, alcohol swabs, and a sharps disposal container.

Wash your hands thoroughly with soap and water.

Preparation of the Injection:

Check the vial of estradiol enanthate for any signs of damage or contamination. If the solution appears discolored or contains particles, do not use it.

Clean the rubber stopper of the vial with an alcohol swab.

Draw air into the syringe equal to the amount of estradiol enanthate you will be withdrawing from the vial.

Insert the needle into the vial and inject the air, which helps to equalize the pressure within the vial.

Withdrawing the Medication:

Invert the vial and hold it securely.

Draw the desired amount of estradiol enanthate into the syringe by pulling back on the plunger until the correct dosage is reached.

Tap the syringe to remove any air bubbles and push the plunger slightly to expel any air if necessary.

Injection Technique:

Choose an injection site, typically the gluteal muscle (buttocks) or the thigh.

Clean the injection site with an alcohol swab and allow it to dry.

Hold the syringe like a dart at a 90-degree angle to the skin.

Insert the needle quickly and firmly into the muscle tissue.

Slowly push the plunger to inject the medication.

Withdraw the needle and apply gentle pressure to the injection site with a clean cotton ball or gauze pad.

Post-Injection Care:

Dispose of the used needle and syringe in a sharps disposal container.

Apply a bandage if necessary.

Wash your hands again with soap and water.

General Considerations:

Rotate injection sites to prevent tissue damage and discomfort.

Adhere to the prescribed dosage and dosing schedule provided by your healthcare provider.

Regularly monitor hormone levels and follow-up with your healthcare provider for adjustments to your treatment regimen if needed.

It’s crucial to receive proper training and guidance from a healthcare professional before administering estradiol enanthate injections or any other medication. They can provide personalized instructions and address any questions or concerns you may have regarding your hormone therapy.

*Please note that these injections are not available in all countries (eg UK)
**Self-administration should only happen after proper training. You can book your injection training session here:

Updated on April 10, 2024

Was this article helpful?

Related Articles

Request an article
If you would like some knowledge added to our knowledge base, send your suggestions here.
Request Knowledge